Recombinant human Carboxypeptidase A4/CPA4 protein
CPA4, also known as carboxypeptidase A4, is a secreted, zinc-dependent metallocarboxypeptidase that removes the C-terminal amino acid from peptides having a free C-terminal carboxyl group. CPA4 is synthesized as zymogens that are activated by proteolytic cleavage. Recombinant human CPA4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Carboxypeptidase A4, CPA4, CPA3
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
46.6 kDa (413aa)
Additionnal Information
References & Citations
Huang H., et al. (1999) Cancer Res. 59:2981-2988.; ; Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_057436
Uniprot Accession Number
Q9UI42
Species
Human
N Terminal Sequence
Gly17
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