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Recombinant human Carboxypeptidase A4/CPA4 protein

CPA4, also known as carboxypeptidase A4, is a secreted, zinc-dependent metallocarboxypeptidase that removes the C-terminal amino acid from peptides having a free C-terminal carboxyl group. CPA4 is synthesized as zymogens that are activated by proteolytic cleavage. Recombinant human CPA4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Carboxypeptidase A4, CPA4, CPA3

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

46.6 kDa (413aa)

Additionnal Information

Carboxypeptidase A4, CPA4, Carboxypeptidase A4, CPA4, CPA3, ATGP3312-10 µg, ATGP3312-20 µg, ATGP3312-50 µg, ATGP3312-100 µg, ATGP3312-250 µg, ATGP3312-500 µg, ATGP3312-1 mg, ATGP3312-10, ATGP3312-20, ATGP3312-50, ATGP3312-100, ATGP3312-250, ATGP3312-500, ATGP3312-1

References & Citations

Huang H., et al. (1999) Cancer Res. 59:2981-2988.; ; Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_057436

Uniprot Accession Number

Q9UI42

Species

Human

N Terminal Sequence

Gly17

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