Recombinant rat Growth Hormone R/GHR protein

GHR, also known as growth hormone receptor, is a member of the cytokine receptor superfamily. GHR forms a complex with a tyrosine kinase, suggesting involvement of a ligand-activated tyrosine kinase in intracellular signaling by growth hormone (GH) . Binding of GH to its receptor results in dimerization of the receptor, phosphorylation of the tyrosine kinase JAK2 and of the receptor, followed by a cascade of protein phosphorylations. Recombinant rat GHR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GHR, GHR/BP

Expression System

Baculovirus

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.4 kDa (255aa)

Additionnal Information

Growth Hormone GHR, GHR, GHR/BP, ATGP3305-10 µg, ATGP3305-20 µg, ATGP3305-50 µg, ATGP3305-100 µg, ATGP3305-250 µg, ATGP3305-500 µg, ATGP3305-1 mg, ATGP3305-10, ATGP3305-20, ATGP3305-50, ATGP3305-100, ATGP3305-250, ATGP3305-500, ATGP3305-1

References & Citations

Sobrier ML., et al. (1997) J Clin Endocrinol Metab. 82:435-437.; ; Postel-Vinay MC., et al. (1996) Baillieres Clin Endocrinol Metab. 10:323-336.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_058790

Uniprot Accession Number

P16310

Species

Rat

N Terminal Sequence

Phe19

AA Sequence

FPGSGATPAT LGKASPVLQR INPSLRESSS GKPRFTKCRS PELETFSCYW TEGDDHNLKV PGSIQLYYAR RIAHEWTPEW KECPDYVSAG ANSCYFNSSY TSIWIPYCIK LTTNGDLLKE KCFTVDEIVQ PDPPIGLNWT LLNISLPGIR GDIQVSWQPP PSADVLKGWI ILEYEIQYKE VNETKWKTMS PIWSTSVPLY SLRLDKEHEV RVRSRQRSFE KYSEFSEVLR VTFPQMDTLA ACEEDFRLEH HHHHH