Recombinant human 4-1BB/CD137/TNFRSF9 protein
TNFRSF9, also known as tumor necrosis factor receptor superfamily member 9, is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. Recombinant human TNFRSF9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human TNFSF9 (CAT# ATGP0308) is coated at 2 µg/mL (100 ul/well) can bind Human TNFRSF9. The ED50 range ≤ 50 ng/mL.
Product Name Alternative
Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, CD137
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human TNFSF9 (CAT# ATGP0308) . The ED50 range ≤ 50 ng/mL.
Molecular Weight
45.3 kDa (411aa)
Additionnal Information
References & Citations
Litjens NH., et al. (2013) Clin. Exp. Immunol. 174:179-191.; ; Lee SJ., et al. (2013) Eur. J. Immunol. 43:1839-1848.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001552
Uniprot Accession Number
Q07011
Species
Human
N Terminal Sequence
Ala
AA Sequence
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