Recombinant mouse P4HB protein

P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Product Name Alternative

P4HB, ERp59, PDI, Pdia1, Thbp, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, p55

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Molecular Weight

56.1 kDa (498aa)

Additionnal Information

P4HB, P4HB, ERp59, PDI, Pdia1, Thbp, ATGP3297-10 µg, ATGP3297-20 µg, ATGP3297-50 µg, ATGP3297-100 µg, ATGP3297-250 µg, ATGP3297-500 µg, ATGP3297-1 mg, ATGP3297-10, ATGP3297-20, ATGP3297-50, ATGP3297-100, ATGP3297-250, ATGP3297-500, ATGP3297-1