Recombinant mouse Legumaisparaginyl Endopeptidase protein

LGMN, also known as legumain, is a lysosomal cysteine protease found in all mouse tissues, but was particularly abundant in kidney and placenta. It plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases. Over expression of Legumain in tumors is significant for invasion/metastasis. Recombinant mouse LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Legumain, AEP, AI746452, AU022324, Prsc1

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.6 kDa (426aa)

Additionnal Information

Legumain, Asparaginyl Endopeptidase, Legumain, AEP, AI746452, AU022324, Prsc1, ATGP3295-10 µg, ATGP3295-20 µg, ATGP3295-50 µg, ATGP3295-100 µg, ATGP3295-250 µg, ATGP3295-500 µg, ATGP3295-1 mg, ATGP3295-10, ATGP3295-20, ATGP3295-50, ATGP3295-100, ATGP3295-250, ATGP3295-500, ATGP3295-1

References & Citations

Shirahama-Noda K., et al. (2003) J. Biol. Chem. 278:33194-33199.; ; Liu C., et al. (2003) Cancer Res. 63:2957-2964.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_035305

Uniprot Accession Number

O89017

Species

Mouse

N Terminal Sequence

Val18

AA Sequence

VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH