Recombinant human Chitinase 3-like 2/CHI3L2 protein

CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis. Recombinant human CHI3L2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CHI3L2, CHIL2, YKL-39, YKL39

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.9 kDa (372aa)

Additionnal Information

Chitinase3-like2, CHI3L2, CHIL2, YKL-39, YKL39, ATGP3294-10 µg, ATGP3294-20 µg, ATGP3294-50 µg, ATGP3294-100 µg, ATGP3294-250 µg, ATGP3294-500 µg, ATGP3294-1 mg, ATGP3294-10, ATGP3294-20, ATGP3294-50, ATGP3294-100, ATGP3294-250, ATGP3294-500, ATGP3294-1

References & Citations

Hu B., et al. (1996) J. Biol. Chem. 271:19415-19420.; ; Gratchev A., et al. (2008) Biomarker Insights. 3:39-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_003991

Uniprot Accession Number

Q15782

Species

Human

N Terminal Sequence

Tyr27

AA Sequence

YKLVCYFTNW SQDRQEPGKF TPENIDPFLC SHLIYSFASI ENNKVIIKDK SEVMLYQTIN SLKTKNPKLK ILLSIGGYLF GSKGFHPMVD SSTSRLEFIN SIILFLRNHN FDGLDVSWIY PDQKENTHFT VLIHELAEAF QKDFTKSTKE RLLLTAGVSA GRQMIDNSYQ VEKLAKDLDF INLLSFDFHG SWEKPLITGH NSPLSKGWQD RGPSSYYNVE YAVGYWIHKG MPSEKVVMGI PTYGHSFTLA SAETTVGAPA SGPGAAGPIT ESSGFLAYYE ICQFLKGAKI TRLQDQQVPY AVKGNQWVGY DDVKSMETKV QFLKNLNLGG AMIWSIDMDD FTGKSCNQGP YPLVQAVKRS LGSLLEHHHH HH