Recombinant human SORD protein

SORD, also known as sorbitol dehydrogenase, is a member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 15 units/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

Product Name Alternative

SORD1, RDH, SDH, SORDD, XDH

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 15unit/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

Molecular Weight

38.3 kDa (357aa)

Additionnal Information

SORD, SORD1, ATGP3293-10 µg, ATGP3293-20 µg, ATGP3293-50 µg, ATGP3293-100 µg, ATGP3293-250 µg, ATGP3293-500 µg, ATGP3293-1 mg, ATGP3293-10, ATGP3293-20, ATGP3293-50, ATGP3293-100, ATGP3293-250, ATGP3293-500, ATGP3293-1

References & Citations

Baker MA., et al. (2010) Proteomics. 10 (3) :482-95.; ; Sorger D., et al. (2009) Nucl Med Biol. 36 (1) :17-27.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_003095.1

Uniprot Accession Number

Q00796

Species

Human

AA Sequence

MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP