Recombinant human Aldo-keto reductase 1D1/AKR1D1 protein

AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.3 kDa (326aa)

Additionnal Information

Aldo-keto reductase1D1, AKR1D1, 3o5bred, CBAS2, SRD5B1, ATGP3292-10 µg, ATGP3292-20 µg, ATGP3292-50 µg, ATGP3292-100 µg, ATGP3292-250 µg, ATGP3292-500 µg, ATGP3292-1 mg, ATGP3292-10, ATGP3292-20, ATGP3292-50, ATGP3292-100, ATGP3292-250, ATGP3292-500, ATGP3292-1

References & Citations

Moore S A., et al. (1995) J Mol Biol. 249 (1) :195-214; ; Gould S J., et al. (1988) Anal Biochem. 175 (1) :5-13

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_005980

Uniprot Accession Number

P51857

Species

Human

AA Sequence

MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY