Recombinant human Aldo-keto reductase 1D1/AKR1D1 protein
AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.3 kDa (326aa)
Additionnal Information
References & Citations
Moore S A., et al. (1995) J Mol Biol. 249 (1) :195-214; ; Gould S J., et al. (1988) Anal Biochem. 175 (1) :5-13
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_005980
Uniprot Accession Number
P51857
Species
Human
AA Sequence
Frequently Asked Questions
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