Recombinant human ASPA protein

ASPA also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, ASPA functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

ACY2, ASP

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.7 kDa (313aa)

Additionnal Information

ASPA, ACY2, ASP, ATGP3289-10 µg, ATGP3289-20 µg, ATGP3289-50 µg, ATGP3289-100 µg, ATGP3289-250 µg, ATGP3289-500 µg, ATGP3289-1 mg, ATGP3289-10, ATGP3289-20, ATGP3289-50, ATGP3289-100, ATGP3289-250, ATGP3289-500, ATGP3289-1

References & Citations

Le Coq., et al. (2006) Biochemistry 45: 5878-5884.; ; Le Coq., et al. (2008) Biochemistry 47: 3484-3492.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_000040

Uniprot Accession Number

P45381

Species

Human

AA Sequence

MTSCHIAEEH IQKVAIFGGT HGNELTGVFL VKHWLENGAE IQRTGLEVKP FITNPRAVKK CTRYIDCDLN RIFDLENLGK KMSEDLPYEV RRAQEINHLF GPKDSEDSYD IIFDLHNTTS NMGCTLILED SRNNFLIQMF HYIKTSLAPL PCYVYLIEHP SLKYATTRSI AKYPVGIEVG PQPQGVLRAD ILDQMRKMIK HALDFIHHFN EGKEFPPCAI EVYKIIEKVD YPRDENGEIA AIIHPNLQDQ DWKPLHPGDP MFLTLDGKTI PLGGDCTVYP VFVNEAAYYE KKEAFAKTTK LTLNAKSIRC CLH