Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein
AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
36.7 kDa (323aa)
Additionnal Information
References & Citations
Zhang Y., et al. (2000) J. Mol. Endocrinol. 25 (2) :221-8.; ; Zhang Y., et al. (2009) Mol Cell Endicrinol. 298 (1-2) :76-83.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 20% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001344
Uniprot Accession Number
Q04828
Species
Human
AA Sequence
Frequently Asked Questions
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