Recombinant mouse Retinol Binding Protein 4/RBP4 protein
RBP4, also known as retinol-binding protein 4, is the specific carrier for retinol, and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. This protein delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin and prevents its loss by filtration through the kidney glomeruli. Recombinant mouse RBP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Plasma retinol-binding protein, PRBP, RBP
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.4 kDa (191aa)
Additionnal Information
References & Citations
Muenzner M., et al. (2013) Mol. Cell. Biol. 33 (20) :4068-4082.; ; Berry DC., et al. (2013) J. Biol. Chem. 288 (34) :24528-24539.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_035385
Uniprot Accession Number
Q00724
Species
Mouse
AA Sequence
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