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Recombinant mouse Ces2e protein

CES2E, also known as pyrethroid hydrolase Ces2e, is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it is widely distributed in cells especially in mammalian liver. It is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Recombinant mouse CES2E, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 30 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.

Product Name Alternative

9030624L02Rik, Ces5, Ces2e

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 30unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.

Molecular Weight

60.5 kDa (541aa)

Additionnal Information

Ces2e, 9030624L02Rik, Ces5, Ces2e, ATGP3273-10 µg, ATGP3273-20 µg, ATGP3273-50 µg, ATGP3273-100 µg, ATGP3273-250 µg, ATGP3273-500 µg, ATGP3273-1 mg, ATGP3273-10, ATGP3273-20, ATGP3273-50, ATGP3273-100, ATGP3273-250, ATGP3273-500, ATGP3273-1

References & Citations

Stok JE., et al. (2004) J Biol Chem. 279:29863-29869.; ; Holmes RS., et al. (2008) Comp Biochem Physiol Part D Genomics Proteomics. 3:196-204.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_766347.1

Uniprot Accession Number

Q8BK48

Species

Mouse

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