Recombinant human Cystatin E/M protein

CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The IC50 value is < 20 nm. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Product Name Alternative

Cystatin-M, Cystatin E/M

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

The IC50 value is < 20 nm. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Molecular Weight

15.9 kDa (142aa) confirmed by MALDI-TOF

Additionnal Information

Cystatin E, M, Cystatin-M, Cystatin E/M, ATGP1146, ATGP3263-10 µg, ATGP3263-20 µg, ATGP3263-50 µg, ATGP3263-100 µg, ATGP3263-250 µg, ATGP3263-500 µg, ATGP3263-1 mg, ATGP3263-10, ATGP3263-20, ATGP3263-50, ATGP3263-100, ATGP3263-250, ATGP3263-500, ATGP3263-1