Recombinant human Cystatin E/M protein
CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Cystatin-M, Cystatin E/M
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
15.9 kDa (142aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ni J., et al. (1997) J Biol Chem. 272 (16) :10853-8.; ; Saitoh E., et al. (1988) Biol Chem Hoppe Seyler. 369:191-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Cardiovascular
NCBI Accession Number
NP_001314.1
Uniprot Accession Number
Q15828
Species
Human
AA Sequence
RPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM
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