Recombinant human Carbonic Anhydrase 8/CA8 protein

CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, this protein lacks carbonic anhydrase activity (i. e., the reversible hydration of carbon dioxide) . It continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3) . Recombinant human CA8 protein fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Product Name Alternative

Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP, CA8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Molecular Weight

35.5 kDa (314aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Carbonic Anhydrase VIII, Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP, ATGP3242-10 µg, ATGP3242-20 µg, ATGP3242-50 µg, ATGP3242-100 µg, ATGP3242-250 µg, ATGP3242-500 µg, ATGP3242-1 mg, ATGP3242-10, ATGP3242-20, ATGP3242-50, ATGP3242-100, ATGP3242-250, ATGP3242-500, ATGP3242-1

References & Citations

Turkmen S., et al. (2009) PLoS Genet. 5:E1000487-E1000487.; ; Skaggs L.A., et al. (1993) Gene. 126:291-292.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_004047

Uniprot Accession Number

P35219

Species

Human

AA Sequence

MADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ