Recombinant human Carbonic Anhydrase 1/CA1 protein

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+) . Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Product Name Alternative

Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Molecular Weight

31.0 kDa (281aa) confirmed by MALDI-TOF

Additionnal Information

Carbonic Anhydrase I, CA1, CA-I, CAB, ATGP3241-10 µg, ATGP3241-20 µg, ATGP3241-50 µg, ATGP3241-100 µg, ATGP3241-250 µg, ATGP3241-500 µg, ATGP3241-1 mg, ATGP3241-10, ATGP3241-20, ATGP3241-50, ATGP3241-100, ATGP3241-250, ATGP3241-500, ATGP3241-1

References & Citations

Nogradi A., et al. (1998) . Am J Pathol. 153:1-4.; ; Ferry JG., et al. (1999) . Proc Natl Acad Sci U S A. 96 (26) :15184-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001122301

Uniprot Accession Number

P00915

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F