Recombinant E.coli Carbonic anhydrase 2/CA2 protein

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+) . Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Product Name Alternative

Carbonate dehydratase, CAN, ECK0125, JW0122, yadF

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Molecular Weight

27.2 kDa (240aa) confirmed by MALDI-TOF

Additionnal Information

YadF, LAMP4, JW0122, GP110, ECK0125, CD68, Carbonic anhydrase2, Carbonic Anhydrase I, Carbonic anhydrase B., Carbonic anhydrase 1, Carbonate dehydratase I, Carbonate dehydratase, Car1, Car 1, CAN, CAI, CA1, CA I, CA 1, ATGP3240-10 µg, ATGP3240-20 µg, ATGP3240-50 µg, ATGP3240-100 µg, ATGP3240-250 µg, ATGP3240-500 µg, ATGP3240-1 mg, ATGP3240-010, ATGP3240-020, ATGP3240-050, ATGP3240-100, ATGP3240-250, ATGP3240-500, ATGP3240-01M

References & Citations

Lindskog S., et al : (1997) Pharmacol Ther.74 (1) :1-20.; ; Sawaya MR., et al (2006) J Biol Chem. 281 (11) :7546-55.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_414668

Uniprot Accession Number

P61517

Species

E.coli

AA Sequence

MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK