Recombinant e.coli idnk protein

D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. Recombinant E. coli idnk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.

Product Name Alternative

D-gluconate kinase thermosensitive, D-gluconate kinase, thermosensitive, ECK4261, gntV, JW4225

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 80unit/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.

Molecular Weight

23.4 kDa (210aa) confirmed by MALDI-TOF

Additionnal Information

Idnk, D-gluconate kinase thermosensitive, D-gluconate kinase, thermosensitive, ECK4261, gntV, JW4225, ATGP3239-10 µg, ATGP3239-20 µg, ATGP3239-50 µg, ATGP3239-100 µg, ATGP3239-250 µg, ATGP3239-500 µg, ATGP3239-1 mg, ATGP3239-10, ATGP3239-20, ATGP3239-50, ATGP3239-100, ATGP3239-250, ATGP3239-500, ATGP3239-1

References & Citations

Coppo P., et al. (2006) J Haematol. 134: 171-179.; ; Zheng X., et al. (2006) BMC cancer. 7: 262.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418689

Uniprot Accession Number

P39208

Species

E.coli

AA Sequence

MAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE