Recombinant human 6-Phosphogluconate Dehydrogenase/PGD protein

PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

Product Name Alternative

6-phosphogluconate dehydrogenase, Decarboxylating, PGDH, Phosphogluconate dehydrogenase, 6PGD

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 10unit/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

Molecular Weight

55.3 kDa (503aa)

Additionnal Information

6-Phosphogluconate Dehydrogenase, PGD, Phosphogluconate dehydrogenase, 6PGD, ATGP3238-10 µg, ATGP3238-20 µg, ATGP3238-50 µg, ATGP3238-100 µg, ATGP3238-250 µg, ATGP3238-500 µg, ATGP3238-1 mg, ATGP3238-10, ATGP3238-20, ATGP3238-50, ATGP3238-100, ATGP3238-250, ATGP3238-500, ATGP3238-1

References & Citations

Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107 (7) :3198-203.; ; Tagen M., et al. (2009) J Immunol. 183 (10) :6313-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002622

Uniprot Accession Number

P52209

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA