Recombinant human 6-Phosphogluconate Dehydrogenase/PGD protein
PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
6-phosphogluconate dehydrogenase, Decarboxylating, PGDH, Phosphogluconate dehydrogenase, 6PGD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
55.3 kDa (503aa)
Additionnal Information
References & Citations
Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107 (7) :3198-203.; ; Tagen M., et al. (2009) J Immunol. 183 (10) :6313-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_002622
Uniprot Accession Number
P52209
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Recombinant Escherichia coli UPF0442 protein yjjB (yjjB), partial
