Recombinant human D amino acid oxidase/DAO protein
D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
D-amino acid oxidase, DAMOX, DAO, OXDA, DAAO
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
41.6 kDa (367aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kawazoe T., et al. (2006) . Protein Sci. 15 (12) :2708-17.; ; Chassande O., et al. (1994) . J Biol Chem. 269 (20) :14484-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT
Scientific Category
Redox Proteins
NCBI Accession Number
NP_001908
Uniprot Accession Number
P14920
Species
Human
AA Sequence
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