Recombinant human D amino acid oxidase/DAO protein

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 3.5 units/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

Product Name Alternative

D-amino acid oxidase, DAMOX, DAO, OXDA, DAAO

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 3.5unit/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

Molecular Weight

41.6 kDa (367aa) confirmed by MALDI-TOF

Additionnal Information

DAAO, D-amino acid oxidase, DAMOX, DAO, OXDA, ATGP3237-10 µg, ATGP3237-20 µg, ATGP3237-50 µg, ATGP3237-100 µg, ATGP3237-250 µg, ATGP3237-500 µg, ATGP3237-1 mg, ATGP3237-10, ATGP3237-20, ATGP3237-50, ATGP3237-100, ATGP3237-250, ATGP3237-500, ATGP3237-1

References & Citations

Kawazoe T., et al. (2006) . Protein Sci. 15 (12) :2708-17.; ; Chassande O., et al. (1994) . J Biol Chem. 269 (20) :14484-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT

Scientific Category

Redox Proteins

NCBI Accession Number

NP_001908

Uniprot Accession Number

P14920

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL