Recombinant E.coli D-lactate dehydrogenase/LDHA protein

Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Product Name Alternative

NAD-dependent, JW1375, htpH, hslI, hslF, Fermentative D-lactate dehydrogenase, ECK1377

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Molecular Weight

39.1 kDa (353aa) confirmed by MALDI-TOF

Additionnal Information

D-lactate dehydrogenase, ldhA, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10 µg, ATGP3236-20 µg, ATGP3236-50 µg, ATGP3236-100 µg, ATGP3236-250 µg, ATGP3236-500 µg, ATGP3236-1 mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1

References & Citations

Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1) : 13-22.; ; Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11) : 25026-25037.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_415898.1

Uniprot Accession Number

P52643

Species

E.coli

AA Sequence

MKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV