Recombinant E.coli D-lactate dehydrogenase/LDHA protein
Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Product Name Alternative
NAD-dependent, JW1375, htpH, hslI, hslF, Fermentative D-lactate dehydrogenase, ECK1377
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Molecular Weight
39.1 kDa (353aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1) : 13-22.; ; Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11) : 25026-25037.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_415898.1
Uniprot Accession Number
P52643
Species
E.coli
AA Sequence
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