Recombinant human FBP2 protein

FBP2 belongs to the FBPase class 1 family. The protein is a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Recombinant human FBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,500 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

Product Name Alternative

Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 1,500pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

Molecular Weight

39.0 kDa (362aa) confirmed by MALDI-TOF

Additionnal Information

FBP2, Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2, ATGP3235-10 µg, ATGP3235-20 µg, ATGP3235-50 µg, ATGP3235-100 µg, ATGP3235-250 µg, ATGP3235-500 µg, ATGP3235-1 mg, ATGP3235-10, ATGP3235-20, ATGP3235-50, ATGP3235-100, ATGP3235-250, ATGP3235-500, ATGP3235-1

References & Citations

Gizak A, Maciaszczyk E, et al. (2008) . Proteins. 72 (1) :209-16.; ; Rakus D, Maciaszczyk E, et al. (2005) . FEBS Lett. 579 (25) :5577-81.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_003828

Uniprot Accession Number

O00757

Species

Human

AA Sequence

MTDRSPF ETDMLTLTRY VMEKGRQAKG TGELTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS