Recombinant human FBP2 protein
FBP2 belongs to the FBPase class 1 family. The protein is a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Recombinant human FBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
39.0 kDa (362aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gizak A, Maciaszczyk E, et al. (2008) . Proteins. 72 (1) :209-16.; ; Rakus D, Maciaszczyk E, et al. (2005) . FEBS Lett. 579 (25) :5577-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_003828
Uniprot Accession Number
O00757
Species
Human
AA Sequence
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