Recombinant mouse Renin protein
REN1, also known as Renin-1, is a member of the peptidase A1 family. It is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. Androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. This protein is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Also, REN1 was found to be co localized with the lysosomal marker, beta-glucuronidase, by double-fluorescent labeling. Recombinant mouse REN1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ren1, Ren, Ren-A, Ren1c, Ren1d, Rn-1, Rnr, Angiotensinogenase, Kidney renin
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
42.5 kDa (387aa)
Additionnal Information
References & Citations
Ramkumar N., et al. (2013) Am. J. Hypertens. 26 (8) :965-972.; ; Bandulik S., et al. (2013) Endocrinology 154 (8) :2712-2722.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_112469
Uniprot Accession Number
P06281
Species
Mouse
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