Recombinant human GSK-3 beta protein
GSK3B, also known as glycogen synthase kinase-3 beta, acts as a negative regulator in the hormonal control of glucose homeostasis. Also, this protein has diverse functions as Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Recombinant human GSK3B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
GSK-3 beta, Serine/threonine-protein kinase GSK3B
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.5 kDa (426aa)
Additionnal Information
References & Citations
Desai SS., et al. (2014) J. Biol. Chem. 289 (9) :5386-5398.; ; Cheng DD., et al. (2014) Biochem. Biophys. Res. Commun. 443 (2) :598-603.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 0.5mM PMSFand 30% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_001139628.1
Uniprot Accession Number
P49841
Species
Human
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