Recombinant human mesotrypsin/PRSS3 protein

PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. Recombinant human PRSS3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Brain trypsinogen, Mesotrypsin, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin-3, Trypsin III, Trypsin IV, PRSS4, TRY3, TRY4

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.3 kDa (233aa)

Additionnal Information

Trypsin3, PRSS3, MTG, PRSS4, T9, TRY3, TRY4, ATGP3220-10 µg, ATGP3220-20 µg, ATGP3220-50 µg, ATGP3220-100 µg, ATGP3220-250 µg, ATGP3220-500 µg, ATGP3220-1 mg, ATGP3220-10, ATGP3220-20, ATGP3220-50, ATGP3220-100, ATGP3220-250, ATGP3220-500, ATGP3220-1

References & Citations

Jiang G., et al. (2010) Gut 59 (11) :1535-1544.; ; Rosendahl J., et al. (2010) Pancreatology 10 (2-3) :243-249.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_031369

Uniprot Accession Number

P35030

Species

Human

AA Sequence

ADPIVGGYTC EENSLPYQVS LNSGSHFCGG SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN RDTLDNDIML IKLSSPAVIN ARVSTISLPT APPAAGTECL ISGWGNTLSF GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANSHHH HHH