Recombinant rat CNTF protein
CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Rat CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤40 ng/mL.
Expression System
E.coli
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤40 ng/mL.
Molecular Weight
25.2 kDa (223aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Linker R., et al. (2009) Crit Rev Immunol. 29 (1) :43-68.; ; Stahl N., et al. (1994) J Neurobiol. 25 (11) :1454-66.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_037298
Uniprot Accession Number
P20294
Species
Rat
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
