Recombinant mouse WIF-1 protein

WIF1, also known as wnt inhibitory factor 1, is a secreted protein that binds to Wnt proteins and inhibits their activities. This protein signaling plays a pivotal role in skeletal development and in the control of cartilage and bone turnover. Also, WIF1 is present in fish, amphibia and mammals, and is expressed during Xenopus and zebrafish development in a complex pattern that includes paraxial presomitic mesoderm, notochord, branchial arches and neural crest derivatives. Recombinant mouse WIF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Wif-1, WIF-1, AW107799

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

39.4 kDa (359aa)

Additionnal Information

WIF1, Wif-1, WIF-1, AW107799, ATGP3217-10 µg, ATGP3217-20 µg, ATGP3217-50 µg, ATGP3217-100 µg, ATGP3217-250 µg, ATGP3217-500 µg, ATGP3217-1 mg, ATGP3217-10, ATGP3217-20, ATGP3217-50, ATGP3217-100, ATGP3217-250, ATGP3217-500, ATGP3217-1

References & Citations

Park JH., et al. (2014) Dev. Biol. 386 (1) :227-236.; ; Stock M., et al. (2013) Arthritis Rheum. 65 (9) :2310-2322.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.5) containing 1mM DTT, 1mM PMSF, 30% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036045

Uniprot Accession Number

Q9WUA1

Species

Mouse

AA Sequence

GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH CEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH