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Recombinant human CD31/PECAM1 protein

PECAM1, also known as platelet endothelial cell adhesion molecule, induces susceptibility to atherosclerosis. It prevents phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. The encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. Recombinant human PECAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD31 stimulates cell adhesion of the Jurkat human acute T cell leukemia cells. This effect is more to 40%.

Product Name Alternative

CD31/EndoCAM, CD31, EndoCAM, GPIIA', PECA1, PECAM1, PECAM-1, Platelet endothelial cell adhesion molecule 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells are added to human CD31 coated plates 5 µg/mL. This effect is more to 40%.

Molecular Weight

65.5 kDa (582aa)

Additionnal Information

CD31, PECAM1, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1, ATGP3214-10 µg, ATGP3214-20 µg, ATGP3214-50 µg, ATGP3214-100 µg, ATGP3214-250 µg, ATGP3214-500 µg, ATGP3214-1 mg, ATGP3214-10, ATGP3214-20, ATGP3214-50, ATGP3214-100, ATGP3214-250, ATGP3214-500, ATGP3214-1

References & Citations

Brown S., et al. (2002) Nature. 418:200-203.; ; Dasgupta B., et al. (2009) J Immunol. 182:5041-5051.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000433

Uniprot Accession Number

P16284

Species

Human

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