Recombinant mouse IL-3 protein

IL3, also known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, it also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Recombinant mouse IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Measured in a cell proliferation assay using BaF3 mouse pro-B cells. The ED50 range ≤0.1 ng/mL.

Product Name Alternative

IL-3, Multipotential colony-stimulating factor (multi-CSF), Mast cell growth factor (MCGF), Interleukin 3 (colony-stimulating factor, multiple), MuLTI-CSF, MCGF, Multilineage-colony-stimulating factor, Hematopoietic growth factor, P-cell stimulating factor, Mast-cell growth factor

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells. The ED50 range ≤0.1 ng/mL.

Molecular Weight

16.7 kDa (148aa)

Additionnal Information

IL3, ATGP3211-10 µg, ATGP3211-20 µg, ATGP3211-50 µg, ATGP3211-100 µg, ATGP3211-250 µg, ATGP3211-500 µg, ATGP3211-1 mg, ATGP3211-10, ATGP3211-20, ATGP3211-50, ATGP3211-100, ATGP3211-250, ATGP3211-500, ATGP3211-1

References & Citations

Yokota. T., et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.; ; Miyatake., S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.; ; Knepper T.P., et al. (1992) Biochemistry 31:11651-11659.