Recombinant mouse CD40 Ligand/CD40LG protein

CD40LG, also known as CD40 ligand, belongs to the tumor necrosis factor family. This protein binds to TNFRSF5. CD40LG mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. CD40 ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. Recombinant mouse CD40LG, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CD40 ligand, CD40-L, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, Hyper-IgM syndrome, HIGM1, T-B cell-activating molecule, T-BAM

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.5 kDa (158aa)

Additionnal Information

CD40 Ligand, TNFSF5, ATGP3200-10 µg, ATGP3200-20 µg, ATGP3200-50 µg, ATGP3200-100 µg, ATGP3200-250 µg, ATGP3200-500 µg, ATGP3200-1 mg, ATGP3200-10, ATGP3200-20, ATGP3200-50, ATGP3200-100, ATGP3200-250, ATGP3200-500, ATGP3200-1

References & Citations

Price JD et al., (2014) J. Leukoc. Biol. 95 (2) :325-336.; ; Williams JA et al., (2014) J. Immunol. 192 (2) :630-640.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_035746

Uniprot Accession Number

P27548

Species

Mouse

AA Sequence

MQRGDED PQIAAHVVSE ANSNAASVLQ WAKKGYYTMK SNLVMLENGK QLTVKREGLY YVYTQVTFCS NREPSSQRPF IVGLWLKPSS GSERILLKAA NTHSSSQLCE QQSVHLGGVF ELQAGASVFV NVTEASQVIH RVGFSSFGLL KL