Recombinant human NMNAT-1 protein

NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

Product Name Alternative

Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, PNAT1, NMNAT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 7,000pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

Molecular Weight

36.0 kDa (315aa) confirmed by MALDI-TOF

Additionnal Information

PNAT1, PNAT 1, NMNAT1, NMNAT 1, NMNAT, NMN/NaMN adenylyltransferase1, NMN/NaMN adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase1, Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, ATGP3194-10 µg, ATGP3194-20 µg, ATGP3194-50 µg, ATGP3194-100 µg, ATGP3194-250 µg, ATGP3194-500 µg, ATGP3194-1 mg, ATGP3194-010, ATGP3194-020, ATGP3194-050, ATGP3194-100, ATGP3194-250, ATGP3194-500, ATGP3194-01M

References & Citations

Emanuelli M. et al. (2001) J Biol Chem. 276 (1) :406-12.; ; Zhou T, ., et al. (2002) J Biol Chem. 277 (15) :13148-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT 1mM EDTA

Scientific Category

Neuroscience

NCBI Accession Number

NP_073624

Uniprot Accession Number

Q9HAN9

Species

Human

AA Sequence

MENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK