Recombinant mouse TNF-alpha protein

TNF, also known as tumor necrosis factor isoform 1, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Mouse TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.02 ng/mL.

Molecular Weight

18kDa (162aa)

Additionnal Information

TNF-a, Tnf, DIF, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2, ATGP3184-10 µg, ATGP3184-20 µg, ATGP3184-50 µg, ATGP3184-100 µg, ATGP3184-250 µg, ATGP3184-500 µg, ATGP3184-1 mg, ATGP3184-10, ATGP3184-20, ATGP3184-50, ATGP3184-100, ATGP3184-250, ATGP3184-500, ATGP3184-1, ATGP3184-10 µg, ATGP3184-20 µg, ATGP3184-50 µg, ATGP3184-100 µg, ATGP3184-250 µg, ATGP3184-500 µg, ATGP3184-1 mg, ATGP3184-10, ATGP3184-20, ATGP3184-50, ATGP3184-100, ATGP3184-250, ATGP3184-500, ATGP3184-1

References & Citations

Marafini I., et al. (2015) PLoS One. 10 (5) :e0126291.; ; Zelova H., et al. (2013) Inflamm Res. 62 (7) :641-651.

Other References

Kim MJ, et al. Paeonia lactiflora Root Extract and Its Components Reduce Biomarkers of Early Atherosclerosis via Anti-Inflammatory and Antioxidant Effects In Vitro and In Vivo. (Antioxidants (Basel) . 2021) {https://pubmed.ncbi.nlm.nih.gov/34679642/}