Recombinant human TNFR2/TNFRSF1B protein
TNFRSF1B, also known as tumor necrosis factor receptor superfamily member 1B, mediates most of the metabolic effects of TNF-alpha. The receptor isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Further, high plasma levels of TNFRSF1B were significantly associated with increased incidence of coronary heart disease. Recombinant human TNFRSF1B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human TNFRSF1B inhibit cytotoxicity in the L-929 mouse fibroblast cells in the presence of Human TNF-α. The ED50 range ≤ 0.2 μg/ml.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit cytotoxicity using L-929 mouse fibroblast cells in the presence of Human TNF-α. The ED50 range ≤ 0.2 μg/ml.
Molecular Weight
25.9 kDa (241aa)
Additionnal Information
References & Citations
Maier O et al., (2013) Biochem. Biophys. Res. Commun. 440 (2) :336-341.; ; Govindaraj C et al., (2013) Clin. Immunol. 149 (1) :97-110.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001057.1
Uniprot Accession Number
P20333
Species
Human
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