Recombinant human AST1/GOT1 protein
Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
48.4 kDa (433aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22) : 5293-9.; ; Totan A., et al. (2006) Clin Chem Lab Med. 44 (5) :612-5.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002070
Uniprot Accession Number
P17174
Species
Human
AA Sequence
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