Recombinant rat Profilin 1/PFN1 protein

Pfn1 also known as Profilin-1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant rat Pfn1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Profilin-1, Profilin, Pfn1

Expression System

E.coli

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.5 kDa (164aa) confirmed by MALDI-TOF

Additionnal Information

Profilin-I, ProfilinI, Profilin-1, Profilin1, Profilin I, Profilin 1, PFN-1, PFN1, PFN 1, Pfn, Actin binding protein, ATGP3173-10 µg, ATGP3173-20 µg, ATGP3173-50 µg, ATGP3173-100 µg, ATGP3173-250 µg, ATGP3173-500 µg, ATGP3173-1 mg, ATGP3173-010, ATGP3173-020, ATGP3173-050, ATGP3173-100, ATGP3173-250, ATGP3173-500, ATGP3173-01M

References & Citations

Tamura M., et al (1996) Biochem. Biophys. Res. Commun. 222 (3) :683-7.; ; Cheng YJ., et al (2015) World Gastroenterol. 21 (8) :2323-35.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_071956

Uniprot Accession Number

P62963

Species

Rat

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAGWNA YIDSLMADGT CQDAAIVGYK DSPSVWAAVP GKTFVSITPA EVGVLVGKDR SSFFVNGLTL GGQKCSVIRD SLLQDGEFTM DLRTKSTGGA PTFNVTVTMT AKTLVLLMGK EGVHGGLINK KCYEMASHLR RSQY