Recombinant mouse RKIP/PEBP1 protein

Pebp1 also known as Phosphatidylethanolamine-binding protein 1. The protein Binds with ATP, opioids and phosphatidylethanolamine. Pebp1 exerts inhibitory activity against several serine proteases including thrombin, neuropsin, and chymotrypsin, it also inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Also, targeting Pebp1 levels can delay photoreceptor degeneration, assisting in extending the time-window for treating such rapidly progressing blindness disorder. Recombinant mouse Pebp1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphatidylethanolamine-binding protein 1, HCNP, Pbp, Pbp1, Pbqr, Rkip, prostatic binding protein, Raf kinase inhibitory protein, hippocampal cholinergic neurostimulating peptide

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.2 kDa (210aa) confirmed by MALDI-TOF

Additionnal Information

Rkip, Raf kinase inhibitory protein, Prostatic binding protein, Phosphatidylethanolamine-binding protein 1, Phosphatidylethanolamine binding protein 1, Pbqr, Pbp1, Pbp 1, Pbp, Hippocampal cholinergic neurostimulating peptide, HCNP, ATGP3172-10 µg, ATGP3172-20 µg, ATGP3172-50 µg, ATGP3172-100 µg, ATGP3172-250 µg, ATGP3172-500 µg, ATGP3172-1 mg, ATGP3172-010, ATGP3172-020, ATGP3172-050, ATGP3172-100, ATGP3172-250, ATGP3172-500, ATGP3172-01M

References & Citations

Hengst U., et al. (2001) J Biol Chem. 276 (1) :535-40; ; Subramanian B., et al. (2014) Invest Ophthalmol Vis Sci. 55 (9) :5788-94

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_061346

Uniprot Accession Number

P70296

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAADISQ WAGPLCLQEV DEPPQHALRV DYAGVTVDEL GKVLTPTQVM NRPSSISWDG LDPGKLYTLV LTDPDAPSRK DPKFREWHHF LVVNMKGNDI SSGTVLSDYV GSGPPSGTGL HRYVWLVYEQ EQPLSCDEPI LSNKSGDNRG KFKVETFRKK YNLGAPVAGT CYQAEWDDYV PKLYEQLSGK