Recombinant human alpha-L-Fucosidase 1/FUCA1 protein

FUCA1, also known as tissue alpha-L-fucosidase, is responsible for hydrolyzing the alpha-1, 6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Fucosidosis is an autosomal recessive lysosomal storage disease resulting from absence of alpha-L-fucosidase activity. Recombinant human FUCA1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tissue alpha-L-fucosidase, α-L-fucosidase 1, Tissue alpha-L-fucosidase, a-L-fucosidase 1, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

51.7 kDa (445aa)

Additionnal Information

Tissue a-L-Fucosidase, FUCA1, Tissue alpha-L-fucosidase, ATGP3170-10 µg, ATGP3170-20 µg, ATGP3170-50 µg, ATGP3170-100 µg, ATGP3170-250 µg, ATGP3170-500 µg, ATGP3170-1 mg, ATGP3170-10, ATGP3170-20, ATGP3170-50, ATGP3170-100, ATGP3170-250, ATGP3170-500, ATGP3170-1

References & Citations

Yang M. et al., (1993) Biochim. Biophys. Acta. 1182 (3) :245-249.; ; Seo H.-C. et al., (1994) Hum. Mol. Genet. 3 (11) :2065-2066.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000138

Uniprot Accession Number

P04066

Species

Human

AA Sequence

VRRAQPPRRY TPDWPSLDSR PLPAWFDEAK FGVFIHWGVF SVPAWGSEWF WWHWQGEGRP QYQRFMRDNY PPGFSYADFG PQFTARFFHP EEWADLFQAA GAKYVVLTTK HHEGFTNWPS PVSWNWNSKD VGPHRDLVGE LGTALRKRNI RYGLYHSLLE WFHPLYLLDK KNGFKTQHFV SAKTMPELYD LVNSYKPDLI WSDGEWECPD TYWNSTNFLS WLYNDSPVKD EVVVNDRWGQ NCSCHHGGYY NCEDKFKPQS LPDHKWEMCT SIDKFSWGYR RDMALSDVTE ESEIISELVQ TVSLGGNYLL NIGPTKDGLI VPIFQERLLA VGKWLSINGE AIYASKPWRV QWEKNTTSVW YTSKGSAVYA IFLHWPENGV LNLESPITTS TTKITMLGIQ GDLKWSTDPD KGLFISLPQL PPSAVPAEFA WTIKLTGVKH HHHHH