Recombinant human alpha-FetoproteiFP protein

AFP, also known as alpha-fetoprotein, is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. This protein binds to copper, nickel, fatty acids and bilirubin. The main function of this is to prevent the virilization of female fetuses. As it does not bind estrogen, its function in humans is less clear. Recombinant human AFP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-fetoprotein, AFPD, FETA, HPAFP

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

67.5 kDa (600aa)

Additionnal Information

AFP, Alpha-fetoprotein, AFPD, FETA, HPAFP, ATGP3169-10 µg, ATGP3169-20 µg, ATGP3169-50 µg, ATGP3169-100 µg, ATGP3169-250 µg, ATGP3169-500 µg, ATGP3169-1 mg, ATGP3169-10, ATGP3169-20, ATGP3169-50, ATGP3169-100, ATGP3169-250, ATGP3169-500, ATGP3169-1

References & Citations

Tomasi TB Jr., (1997) Annu Rev Med. 28:453-465.; ; Elbrecht A., et al. (1992) Science. 255:467-470.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001125.1

Uniprot Accession Number

P02771

Species

Human

AA Sequence

RTLHRNE YGIASILDSY QCTAEISLAD LATIFFAQFV QEATYKEVSK MVKDALTAIE KPTGDEQSSG CLENQLPAFL EELCHEKEIL EKYGHSDCCS QSEEGRHNCF LAHKKPTPAS IPLFQVPEPV TSCEAYEEDR ETFMNKFIYE IARRHPFLYA PTILLWAARY DKIIPSCCKA ENAVECFQTK AATVTKELRE SSLLNQHACA VMKNFGTRTF QAITVTKLSQ KFTKVNFTEI QKLVLDVAHV HEHCCRGDVL DCLQDGEKIM SYICSQQDTL SNKITECCKL TTLERGQCII HAENDEKPEG LSPNLNRFLG DRDFNQFSSG EKNIFLASFV HEYSRRHPQL AVSVILRVAK GYQELLEKCF QTENPLECQD KGEEELQKYI QESQALAKRS CGLFQKLGEY YLQNAFLVAY TKKAPQLTSS ELMAITRKMA ATAATCCQLS EDKLLACGEG AADIIIGHLC IRHEMTPVNP GVGQCCTSSY ANRRPCFSSL VVDETYVPPA FSDDKFIFHK DLCQAQGVAL QTMKQEFLIN LVKQKPQITE EQLEAVIADF SGLLEKCCQG QEQEVCFAEE GQKLISKTRA ALGV