Recombinant human alpha-Actinin 1/ACTN1 protein

ACTN1 also known as alpha-actinin-1 isoform a. ACTN1 functions as an actin-binding-protein. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration. Recombinant human ACTN1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-actinin-1 isoform a, Actinin alpha 1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

31.4 kDa (274aa) confirmed by MALDI-TOF

Additionnal Information

Non-muscle alpha-actinin-1, F-actin cross-linking protein, Alpha-actinin-1 isoform a, Alpha-actinin cytoskeletal isoform, ACTN1, Actinin alpha 1, a-Actinin1, ATGP3162-10 µg, ATGP3162-20 µg, ATGP3162-50 µg, ATGP3162-100 µg, ATGP3162-250 µg, ATGP3162-500 µg, ATGP3162-1 mg, ATGP3162-010, ATGP3162-020, ATGP3162-050, ATGP3162-100, ATGP3162-250, ATGP3162-500, ATGP3162-01M

References & Citations

Hamill KJ., et al. (2014) Journal of Invest Dermatology. 135 (4) :1043-52; ; Kunishima S., et al. (2013) Am J Hum Genet. 92 (3) :431-8

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001123476

Uniprot Accession Number

P12814

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMDHYD SQQTNDYMQP EEDWDRDLLL DPAWEKQQRK TFTAWCNSHL RKAGTQIENI EEDFRDGLKL MLLLEVISGE RLAKPERGKM RVHKISNVNK ALDFIASKGV KLVSIGAEEI VDGNVKMTLG MIWTIILRFA IQDISVEETS AKEGLLLWCQ RKTAPYKNVN IQNFHISWKD GLGFCALIHR HRPELIDYGK LRKDDPLTNL NTAFDVAEKY LDIPKMLDAE DIVGTARPDE KAIMTYVSSF YHAF