Recombinant rat ALT1/GPT1 protein

Gpt also known as Alanine aminotransferase 1, is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate. The Gpt is widely distributed in various tissues from animals and even in some kind of plants. It is suggested that c-ALT is associated to the utilization of pyruvate in glycolysis and m-ALT is involved in the conversion of alanine to pyruvate for gluconeogenesis. Recombinant rat Gpt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 60units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

Product Name Alternative

Alanine aminotransferase 1

Expression System

E.coli

Antigen Species

Rat

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 60unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

Molecular Weight

57.5 kDa (519aa)

Additionnal Information

ALT1, GPT1, Alanine aminotransferase 1, ATGP3161-10 µg, ATGP3161-20 µg, ATGP3161-50 µg, ATGP3161-100 µg, ATGP3161-250 µg, ATGP3161-500 µg, ATGP3161-1 mg, ATGP3161-10, ATGP3161-20, ATGP3161-50, ATGP3161-100, ATGP3161-250, ATGP3161-500, ATGP3161-1

References & Citations

Yang RZ., et al. (2009) Hepatology. 49 (2) :598-607.; ; Ishiguro M., et al. (1991) Biochemistry. 30 (43) :10451-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_112301

Uniprot Accession Number

P25409

Species

Rat

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASRVND QSQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS