Recombinant human PP2A alpha/PPP2R1A protein

PPP2R1A also known as Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform, is a member of the PPP family of phosphatases, regulates a variety of essential cellular processes. PPP2R1A phosphorylation is an in vivo mechanism for regulation of the PP2A signaling complex and increased PP2A activity in heart failure. Recombinant human PPP2R1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein phosphatase 2 scaffold subunit Aalpha, Protein phosphatase 2A, Regulatory subunit A, alpha isoform, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PR65A, PP2A-Aalpha, PP2AA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

67.7 kDa (612aa)

Additionnal Information

PPP2R1A, Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PP2A-Aalpha, PP2AAALPHA, PR65A, ATGP3160-10 µg, ATGP3160-20 µg, ATGP3160-50 µg, ATGP3160-100 µg, ATGP3160-250 µg, ATGP3160-500 µg, ATGP3160-1 mg, ATGP3160-10, ATGP3160-20, ATGP3160-50, ATGP3160-100, ATGP3160-250, ATGP3160-500, ATGP3160-1

References & Citations

Yang R., et al. (2013) PLoS One. 8 (10) :e77285.; ; Li H H., et al. (2007) EMBO J. 26 (2) : 902-914.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055040

Uniprot Accession Number

P30153

Species

Human

AA Sequence

MAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDA NQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA