Recombinant human TRACP/PAP/ACP5 protein

ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 5,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Product Name Alternative

Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 5,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Molecular Weight

35.1 kDa (310aa)

Additionnal Information

TRACP, PAP, ACP5, Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP, ATGP3155-10 µg, ATGP3155-20 µg, ATGP3155-50 µg, ATGP3155-100 µg, ATGP3155-250 µg, ATGP3155-500 µg, ATGP3155-1 mg, ATGP3155-10, ATGP3155-20, ATGP3155-50, ATGP3155-100, ATGP3155-250, ATGP3155-500, ATGP3155-1