Recombinant human QPCT protein

QPCT, also known as glutaminyl-peptide cyclotransferase, is responsible for the biosynthesis of pyroglutamyl peptides. This protein has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. It catalyzes N-terminal pyroglutamate formation. In vitro, it catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. Recombinant human QPCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutaminyl-peptide cyclotransferase, GCT, QC, sQC

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.7 kDa (339aa)

Additionnal Information

QPCT, Glutaminyl-peptide cyclotransferase, GCT, QC, sQC, ATGP3153-10 µg, ATGP3153-20 µg, ATGP3153-50 µg, ATGP3153-100 µg, ATGP3153-250 µg, ATGP3153-500 µg, ATGP3153-1 mg, ATGP3153-10, ATGP3153-20, ATGP3153-50, ATGP3153-100, ATGP3153-250, ATGP3153-500, ATGP3153-1

References & Citations

Schilling S. et al., (2004) FEBS Lett. 563:191-196.; ; Huang K.F. et al., (2011) J. Biol. Chem. 286:12439-12449.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_036545

Uniprot Accession Number

Q16769

Species

Human

AA Sequence

VSPSASAWPE EKNYHQPAIL NSSALRQIAE GTSISEMWQN DLQPLLIERY PGSPGSYAAR QHIMQRIQRL QADWVLEIDT FLSQTPYGYR SFSNIISTLN PTAKRHLVLA CHYDSKYFSH WNNRVFVGAT DSAVPCAMML ELARALDKKL LSLKTVSDSK PDLSLQLIFF DGEEAFLHWS PQDSLYGSRH LAAKMASTPH PPGARGTSQL HGMDLLVLLD LIGAPNPTFP NFFPNSARWF ERLQAIEHEL HELGLLKDHS LEGRYFQNYS YGGVIQDDHI PFLRRGVPVL HLIPSPFPEV WHTMDDNEEN LDESTIDNLN KILQVFVLEY LHLHHHHHH