Recombinant human TNF-alpha protein
TNF, also known as tumor necrosis factor, is cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Product Name Alternative
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
18.1 kDa (163aa)
Additionnal Information
References & Citations
Friedmann E. et al., (2006) Nat. Cell Biol. 8:843-848.; ; Zhang XM. et al., (1992) J. Biol. Chem. 267:24069-24075.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000585.2
Uniprot Accession Number
P01375
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Lentiviral mouse Gas2 shRNA (UAS) - Lentiviral mouse Gas2 shRNA (UAS, iRFP) (25)
Recombinant Human GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) , partial
Recombinant Human GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) , partial
Recombinant Human GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) , partial
Recombinant Human GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) , partial